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1 h nmr observation of tissue myoglobin: an indicator of cellular oxygenation in vivo
Author(s) -
Jue T.,
Anderson S.
Publication year - 1990
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910130322
Subject(s) - myoglobin , in vivo , extant taxon , oxygenation , oxygen tension , chemistry , oxygen , proton nmr , nuclear magnetic resonance , biophysics , biochemistry , biology , ecology , physics , microbiology and biotechnology , organic chemistry , evolutionary biology , stereochemistry
Cellular oxygen status is a critical parameter. To measure the oxygen tension in vivo , however, demands extant techniques to surmount two experimental difficulties: invasive experimental protocols and imprecise tissue localization. NMR techniques have the potential to overcome these obstacles. Indeed we show in this study that cellular myoglobin is NMR visible and its proximal histidyl NH signal in the deoxy state can be used to monitor changes in cellular oxygenation. Coupled with recent advances in NMR signal localization, this 1 H NMR strategy promises to lead directly to cellular oxygen measurement in specific tissue regions in vivo.