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Inhibition of lymphocyte stimulation by shift reagents
Author(s) -
Sze Daniel Y.,
Corbelletta Nancy L.,
Shochat Stephen J.,
Jardetzky Oleg
Publication year - 1990
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910130104
Subject(s) - reagent , stimulation , chemistry , lanthanide , inert , biophysics , combinatorial chemistry , neuroscience , organic chemistry , biology , ion
Abstract Lanthanide shift reagents have opened a new avenue in the study of membrane biochemistry, but their stabilities and biological reactivities remain questionable. We present evidence that shift reagents are not biologically inert, and that they exhibit the ability to inhibit stimulation of human peripheral lymphocytes at commonly used concentrations. A survey of various mitogens yielded no shift reagent‐resistant modes of stimulation, and a survey of various shift reagents yielded no effective and nontoxic alternatives. Involvement of calcium‐regulating mechanisms was not apparent. The assumption that lanthanide shift reagents used in NMR studies are nondestructive and physiologically innocuous is thus shown to be unwarranted.