Premium
Intrauterine fetal brain NMR spectroscopy: 1 H and 31 P studies in rats
Author(s) -
Nakada Tsutomu,
Kwee Ingrid L.,
Suzuki Nobuyuki,
Houkin Kiyohiro
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910120204
Subject(s) - fetus , nuclear magnetic resonance spectroscopy , chemistry , in utero , in vivo , phosphocreatine , choline , phospholipid , spectroscopy , phosphomonoesters , medicine , intracellular ph , endocrinology , nuclear magnetic resonance , biology , biochemistry , intracellular , pregnancy , membrane , genetics , microbiology and biotechnology , physics , organic chemistry , quantum mechanics , energy metabolism
Fetal brain metabolism was investigated in utero noninvasively using multinuclear nuclear magnetic resonance spectroscopy in rats at two representative prenatal stages: early (17‐18 days) and late (20‐21 days) stages. Phosphorus‐31 ( 31 P) spectroscopy revealed that phosphocreatine is significantly lower in the early stage and increases to the level of early neonates by the late prenatal stage. Intracellular pH at the early stage was found to be strikingly high (7.52 ± 0.21) and decreased to a level similar to that of neonates by the late stage (7.29 ± 0.07). Phosphomonoester levels at both stages were similar to the values reported for early neonates. Water‐suppressed proton ( 1 H) spectroscopy demonstrated a distinctive in vivo fetal brain spectral pattern characterized by low levels of N ‐acetyl aspartate and high levels of taurine. High‐resolution proton spectroscopy and homonuclear chemical‐shift correlate spectroscopy of brain perchloric acid extracts confirmed these in vivo findings. In vitro 31 P spectroscopy of acidified chloroform methanol extracts showed the characteristic membrane phospholipid profiles of fetal brain. The phosphatidylethanolamine (PE)‐to‐phosphatidylcholine (PC) ratio (PE/PC) did not show significant changes between the two stages at 0.40 ± 0.11, a value similar to that of early neonates. © 1989 Academic Press, Inc.