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in vitro NMR characterization of mammalian myocardium: Effect of specimen integrity on relaxation times
Author(s) -
Scholz Thomas D.,
Fleagle Steven R.,
Skorton David J.
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910110310
Subject(s) - relaxometry , t2 relaxation , relaxation (psychology) , chemistry , nuclear magnetic resonance , significant difference , spin–lattice relaxation , nuclear medicine , magnetic resonance imaging , medicine , radiology , spin echo , physics , nuclear quadrupole resonance
We systematically evaluated three different methods of preparing canine myocardium for NMR relaxometry: using whole specimens, specimens sliced into approximately 2‐mm 3 pieces, and specimens minced into pieces <2 mm 3 . NMR relaxation times were determined at 20 MHz. TI relaxation time and water content were not different among the three methods. However, T2 values differed significantly between whole and sliced samples (mean ± SEM = 51.2 ± 2.2 ms (whole) vs 54.3 ± 2.7 ms (sliced); P < 0.05). Minced myocardium showed a similar increase that was not statistically significant. We conclude that tissue preparation methods must be controlled when performing NMR relaxometry studies.