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Study of proton spin‐lattice relaxation variation induced by paramagnetic antibodies
Author(s) -
Macr M. A.,
De Luca F.,
Maraviglia B.,
Polizio F.,
Garreffa G.,
Cavallo S.,
Natali P. G.
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910110302
Subject(s) - gadolinium , relaxation (psychology) , paramagnetism , nuclear magnetic resonance , spin–lattice relaxation , proton , chemistry , monoclonal antibody , spectral line , spin label , antibody , electron paramagnetic resonance , physics , medicine , condensed matter physics , nuclear physics , immunology , organic chemistry , astronomy
A murine anti‐human melanoma monoclonal antibody fragment was labeled with gadolinium and its proton relaxation efficiency compared to controls at frequencies ranging from 2 to 300 MHz. Relaxation time variations were about 30–40% in 10–15 μM solutions. The labeled fragment showed proton relaxation enhancement relative to free gadolinium, while preserving its immunoreactivity. A tentative labeling of a melanoma pellet by means of the fragment, just at the borderline of a minimum expected T 1 variation, gave no detectable diffemm.