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In vivo 31 P nuclear magnetic resonance spectroscopy of rat 9l gliosarcoma treated with BCNU: Dose response of spectral changes
Author(s) -
Grant Steen R.,
Tamargo Rafael J.,
Brem Henry,
Glickson Jerry D.,
Wehrle Janna P.
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910110214
Subject(s) - gliosarcoma , clonogenic assay , in vivo , chemistry , toxicity , nuclear magnetic resonance spectroscopy , nuclear medicine , nuclear magnetic resonance , radiochemistry , cancer research , medicine , cell , biology , biochemistry , glioma , stereochemistry , physics , microbiology and biotechnology , organic chemistry
The 9L gliosarcoma, grown subcutaneously in juvenile Fischer 344 rats, was studied by in vivo 31 P NMR spectroscopy following treatment with 1,3‐bis(2‐chloroethyl)‐ 1‐nitrosourea. Dose‐dependent increases in the proportion of high‐energy phosphates were observed for doses between 10 and 36 mg/kg (from 80% of the LD 10 to greater than the LD 50 ). These doses reduced clonogenic cell survival in a dose‐dependent fashion by as much as 3 log orders and resulted in up to 16 days of growth delay (to pretreatment tumor volume). Increases in high‐energy phosphates (relative to P i ) in the tumor were greater at higher doses despite the higher levels of clonogenic cell killing and the substantial host Systemic toxicity.