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1 H and 31 P NMR measurement of cerebral lactate, high‐energy phosphate levels, and pH in humans during voluntary hyperventilation: associated EEG, capnographic, and doppler findings
Author(s) -
van Rijen P. C.,
Luyten P. R.,
Van der Sprenkel J. W. Berkelbach,
Kraaier V.,
van Huffelen A. C.,
Tulleken C. A. F.,
den Hollander J. A.
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910100204
Subject(s) - hyperventilation , chemistry , intracellular ph , alkalosis , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , nmr spectra database , acidosis , hypocapnia , medicine , analytical chemistry (journal) , spectral line , chromatography , extracellular , biochemistry , hypercapnia , stereochemistry , physics , astronomy
In order to explore the sensitivity of spatially resolved 1 H and 31 P NMR spectroscopy on a whole‐body NMR instrument, cerebral metabolic changes in human volunteers were measured during hyperventilation provocation. During hyperventilation the flow velocity in the middle cerebral artery decreased significantly and the EEG showed a marked increase in slow activity. 1 H NMR spectra revealed an increase in cerebral lactate concentration. 31 P NMR spectra showed no changes in ATP or PCr peak heights, but a shift toward tissue alkalosis was derived from changes in P i chemical shift. During subsequent recovery, lactate concentration decreased and a slight intracellular acidosis was detected. In three experiments broadening of the lactate resonance peak resulted in separation into two components at 1.32 and 1.48 ppm, in which the latter signal possibly arose from alanine. © 1989 Academic press. Inc.