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A model of acute regional myocardial ischemia and reperfusion in the rat
Author(s) -
Sievers R. E.,
Schmiedl U.,
Wolfe C. L.,
Moseley M. E.,
Parmley W. W.,
Brasch R. C.,
Lipton M. J.
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910100203
Subject(s) - ischemia , medicine , occlusion , cardiology , coronary occlusion , in vivo , hemodynamics , magnetic resonance imaging , blood flow , diastole , radiology , blood pressure , microbiology and biotechnology , biology
Studies were conducted in 76 rats to describe and validate a new closed‐chest in vivo model for acute ischemia and reperfusion of the left coronary artery. Radiolabeled microsphere distribution in six rat hearts confirmed a significant reduction in arteriolar flow at the center of the ischemic zone (93% reduction of total myocardial counts compared to nonischemic region, P < 0.01) after 7 min of occlusion. Arteriolar flow returned to control values upon reperfusion. While hemodynamic parameters in 10 ratsduring 7 min of occlusion and 7 min of reperfusion were monitored, end diastolic pressures increased significantly ( P < 0.01) during occlusion. Finally, the utility of this rat model was demonstrated in a study of contrast enhanced magnetic resonance imaging ( N =4). Normal myocardium could not be differentiated from acutely ischemic myocardium on noncontrast‐enhanced MR images. After 5 min of myocardial ischemia and following contrast administration (albumin‐Gd‐DTPA), the ischemic zone appeared less enhanced than normal myocardium. Upon release ofthe occluder the left ventricular free wall once again yielded a homogeneous signal similar to that of the normal myocardium. © 1989 Academic Press, Inc.