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Transmural metabolite distribution in regional myocardial ischemia as studied with 31 p NMR
Author(s) -
Robitaille PierreMarie,
Lew Brian,
Merkle Hellmut,
Sublett Eugene,
Lindstrom Paul,
From Arthur H. L.,
Garwood Michael,
Bache Robert J.,
Uǧubil Kâmil
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910100110
Subject(s) - endocardium , ventricle , nuclear magnetic resonance , chemistry , ischemia , perfusion , carnivora , medicine , cardiology , physics
Phosphorus‐31 nuclear magnetic resonance ( 31 P NMR) has been applied to study the canine heart prior to and during regional myocardial ischemia induced by partial flow reduction in the left anterior descending coronary artery (LAD). NMR data were acquired in a transmural fashion by restricting the signal to a column perpendicular to the heart wall using B 0 gradients and obtaining spectroscopic spatial resolution along the third dimension using the B 1 gradient and adiabatic excitation. With this approach, transmural spectra were accumulated in five separate voxels spanning the wall of the left ventricle from the epicardium to the endocardium. In the normal canine myocardium the levels of high‐energy phosphates CP and ATP were relatively constant throughout the left ventricular wall, with only minor evidence of free inorganic phosphate in any of the transmural voxels. However, during sustained partial occlusion of the LAD, significant regional differences between the epi‐ and the endocardium were noted. The data demonstrate the importance of studying cardiac bioenergetics with transmural differentiation. © 1989 Academic Press, Inc.