A perfusion system developed for  31 p NMR study of melanoma cells at tissue‐like density | Zendy

Minichiello Margaret M. | Zendy; Albert Daniel M. | Zendy; Kolodny Nancy H. | Zendy; Lee MoonSoo | Zendy; Craft Joseph L. | Zendy

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A perfusion system developed for 31 p NMR study of melanoma cells at tissue‐like density

Author(s) -

Minichiello Margaret M.,

Albert Daniel M.,

Kolodny Nancy H.,

Lee MoonSoo,

Craft Joseph L.

Publication year - 1989

Publication title -

magnetic resonance in medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.696

H-Index - 225

eISSN - 1522-2594

pISSN - 0740-3194

DOI - 10.1002/mrm.1910100109

Subject(s) - perfusion , nuclear magnetic resonance , melanoma , nuclear magnetic resonance spectroscopy , chemistry , nuclear medicine , physics , medicine , radiology , cancer research

A perfusion culture system has been developed for 31 P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31 P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage‐dependent cells grew to tissue‐like density. © 1989 Academic Press, Inc.

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