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A perfusion system developed for 31 p NMR study of melanoma cells at tissue‐like density
Author(s) -
Minichiello Margaret M.,
Albert Daniel M.,
Kolodny Nancy H.,
Lee MoonSoo,
Craft Joseph L.
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910100109
Subject(s) - perfusion , nuclear magnetic resonance , melanoma , nuclear magnetic resonance spectroscopy , chemistry , nuclear medicine , physics , medicine , radiology , cancer research
A perfusion culture system has been developed for 31 P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31 P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage‐dependent cells grew to tissue‐like density. © 1989 Academic Press, Inc.