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Pathways for the synthesis of sorbitol from 13 C‐labeled hexoses, pentose, and glycerol in renal papillary tissue
Author(s) -
Jans A. W. H.,
Grunewald R.W.,
Kinne R. K. H.
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910090315
Subject(s) - sorbitol , pentose , glycerol , glyceraldehyde , chemistry , sorbitol dehydrogenase , fructose , biochemistry , aldose reductase , pentose phosphate pathway , polyol pathway , ribose , polyol , dehydrogenase , metabolism , enzyme , glycolysis , organic chemistry , fermentation , polyurethane
Suspensions of rabbit renal papillary tissue were incubated with D‐[ 6‐ 13 C]glucose, D‐[1‐ 13 C]fructose, D‐[1‐ 13 C] ribose, and [2‐ 13 C]glycerol. The perchloric acid extracts of the above incubations were investigated with 13 C NMR spectroscopy. All 13 C‐labeled substrates give rise to 13 C‐labeled D‐sorbitol. D‐[6‐ 13 C]Glucose and D[1‐ 13 C]fructose are converted directly into D‐sorbitol via the aldose reductase and sorbitol dehydrogenase pathway, respectively, whereas D‐[1‐ 13 C] ribose and [2‐ 13 C]glycerol give rise to labeling of the D‐glyceraldehyde pool which on its turn causes a labeling of D‐sorbitol. Label exchanges observed from incubations with glycerol and D‐ribose indicate that the pentose shunt plays a role in this synthesis of D‐sorbitol. © 1989 Academic Press, Inc.