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23 Na NMR measurement of the maximal rate of active sodium efflux from human red blood cells
Author(s) -
Knubovets T. L.,
Revazov A. V.,
Sibeldina L. A.,
Eichhoff U.
Publication year - 1989
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910090211
Subject(s) - extracellular , efflux , ouabain , chemistry , sodium , intracellular , reagent , isotopes of sodium , ion , biophysics , chromatography , analytical chemistry (journal) , biochemistry , biology , organic chemistry
The method for 23 Na NMR measurement of the maximal rate of active Na + efflux from human red blood cells (RBC) is proposed. The nonpenetrating paramagnetic shift reagent (SR) bis(tripolyphosphate)dysprosium(III) complex is used to distinguish extracellular Na + ions from intracellular. RBC are proved to retain their physiological activity in the presence of SR. Intracellular Na + is shown to be 100% NMR visible. The levels of intracellular and extracellular Na + and K + ions are changed to decrease their concentration gradients across the erythrocyte membrane to make active Na + efflux the only 23 Na NMR measurable process; so the integrated areas of intra‐and extracellular Na + peaks remain invariant throughout the incubation period in the presence of 0.25 m M ouabain, a specific inhibitor of Na + , K + ‐ATPase. The accuracy of the proposed technique is evaluated to be 10%. The maximal Na + efflux is determined to be 10.1 ± 1.0 m M /h/liter of Cells. © 1989 Academic Press, Inc.