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Spin probe reduction in cells and tissues
Author(s) -
Kveder Marina,
Sentjurc Marjeta,
Schara Milan
Publication year - 1988
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910080302
Subject(s) - nitroxide mediated radical polymerization , electron paramagnetic resonance , chemistry , spin probe , nuclear magnetic resonance , oxygen , reduction (mathematics) , site directed spin labeling , molecule , paramagnetism , biophysics , organic chemistry , polymer , polymerization , biology , physics , geometry , mathematics , radical polymerization , quantum mechanics
The reduction rates of different nitroxides in rat lung tissue were measured by electron paramagnetic resonance. We confirmed that the reduction rate of nitroxide spin probe molecules is coupled with their structure and transport characteristics. Oxygen was found to slow down the reduction rate of nitroxides in rat lung tissues. On the basis of these findings it can be concluded that most nitroxide properties described for homogeneous systems—cells and tissue homogenates—are also valid for heterogeneous systems, which is important for the application of nitroxides as metabolically active NMR contrast agents. © 1988 Academic Press, Inc.