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Quantitative estimation of lactate in the brain by 1 H NMR
Author(s) -
Williams Stephen R.,
Proctor Edward,
Allen Kathryn,
Gadian David G.,
Crockard H. Alan
Publication year - 1988
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910070405
Subject(s) - phosphocreatine , creatine , nmr spectra database , chemistry , creatine kinase , nuclear magnetic resonance , biology , biochemistry , endocrinology , spectral line , physics , astronomy , energy metabolism
1 H NMR was used to detect lactate accumulation in the intact gerbil brain postmortem. The lactate concentration was estimated from the spectra by comparison to signals from N‐acetylaspartate, creatine + phosphocreatine, and water. The effects of T 2 , phase modulation, and solvent suppression were taken into account. The estimated concentrations were compared to determinations performed on the same brains after extraction. The lactate concentration estimated from the intact brain spectra was between 70 and 90% of the values determined in vitro , on the extracts, depending on the concentration standard used. If N‐acetylaspartate was used as the standard then the proportion of detected lactate (92%) was not significantly different from 100%. © 1988 Academic Press, Inc.