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31 P NMR relaxation time studies of 2,3‐diphosphoglycerate in solution and intact erythrocytes
Author(s) -
Christensen Marianne,
Jacobsen Jens Peter
Publication year - 1988
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910070207
Subject(s) - diphosphoglycerate , chemistry , relaxation (psychology) , spin–lattice relaxation , nuclear magnetic resonance , anisotropy , dipole , nuclear overhauser effect , hemoglobin , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , chromatography , stereochemistry , biochemistry , physics , psychology , social psychology , organic chemistry , quantum mechanics , nuclear quadrupole resonance
The 31 P T 1 spin‐lattice relaxation times and nuclear Overhauser effects of 3‐P and 2‐P in 2,3‐diphosphoglycerate (2,3‐DPG) have been measured in pure water solutions and in suspensions of intact erythrocytes. It was found that extremely careful purification of the water solutions from paramagnetic impurities was necessary in order to obtain reproducible results. The dominant relaxation mechanism in purified samples was shown to be the dipole‐dipole interaction. Contributions from the chemical‐shift anisotropy mechanism were demonstrated to be important at higher magnetic field strengths. Based upon the measurements in water solutions and intact erythrocyte suspensions it was concluded that there could be observed no significant influence of the oxygenation state of hemoglobin on the 31 P T 1 values of 2,3‐DPG. © 1988 Academic Press, Inc.