Premium
Characterization of high‐energy phosphate compounds during reperfusion of the irreversibly injured myocardium using 31 P MRS
Author(s) -
Wendland Michael F.,
White Richard D.,
Derugin Nikita,
Finkbeiner Walter E.,
McNamara Michael T.,
Moseley Michael E.,
Lipton Martin J.,
Higgins Charles B.
Publication year - 1988
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910070205
Subject(s) - phosphocreatine , chemistry , inorganic phosphate , intracellular ph , medicine , ischemia , phosphorus , pi , intensity (physics) , phosphate , high energy phosphate , occlusion , cardiology , intracellular , energy metabolism , biochemistry , physics , organic chemistry , quantum mechanics
Phosphorus‐31 magnetic resonance spectroscopy (MRS) was used to monitor regional changes in high‐energy phosphorus compounds and intracellular pH during 60 min of acute regional ischemia (acute occlusion of left anterior descending artery) and reperfusion in open‐chest cats using a 1.2‐cm two‐turn coil sutured to the myocardium. During the 60‐min ischemic phase, phosphocreatine (PCr) intensity was reduced to 47 ± 4.9% (mean ± SE) of control ( p < 0.01) by 15 min postocclusion while adenosine triphosphate (ATP) intensity decreased more slowly with the decrease (66 ± 5.6%) achieving significance ( p < 0.05) only at 60 min postocclusion. Inorganic phosphate ( P i) increased to a maximum of 397 ± 42% of control ( p < 0.01) while the pH decreased progressively from 7.36 ± 0.02 to 6.02 ± 0.14 ( p < 0.01). After release of occlusion PCr intensity recovered to 86 ± 12% of the initial control value at 15 min postreperfusion but showed a subsequent downward trend to 79 ± 8.8%. The ATP did not recover but tended to decline further during reperfusion. The Pi intensity decreased to 260 ± 38% of control while the pH increased to 7.01 ± 0.23 by 15 min postreperfusion. Thus, the reperfused irreversibly injured myocardium is characterized by persistent depletion of PCr and ATP and elevation of Pi. Phosphorus‐31 MRS provides a nondestructive method for characterizing the reperfused irreversibly damaged myocardium. © 1988 Academic Press, Inc.