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Saturation and inversion transfer studies of creatine kinase kinetics in rabbit skeletal muscle in vivo
Author(s) -
Hsieh Paul S.,
Balaban Robert S.
Publication year - 1988
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910070107
Subject(s) - creatine kinase , creatine , skeletal muscle , kinetics , chemistry , saturation (graph theory) , in vivo , magnetization transfer , phosphate , phosphocreatine , creatine monohydrate , biophysics , enzyme kinetics , biochemistry , anatomy , biology , endocrinology , enzyme , medicine , physics , magnetic resonance imaging , pathology , energy metabolism , active site , alternative medicine , mathematics , placebo , microbiology and biotechnology , quantum mechanics , radiology , combinatorics
The steady‐state kinetics of the creatine kinase reaction in rabbit skeletal muscle in vivo was investigated using inversion and saturation magnetization transfer techniques. Both techniques determined the forward rate of this reaction (creatine phosphate ATP) as approximately 0.3 s −1 . This corresponds to a flux of 10 μmol creatine phosphate/s/g muscle. The saturation transfer technique underestimated the reverse reaction by approximately 56%. This result is likely due to the participation of ATP in other interactions in skeletal muscle not involving creatine phosphate. © 1988 Academic Press, Inc.