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Quantitation of acidosis in neonatal brain tissue using the 31 P NMR resonance peak of phosphoethanolamine
Author(s) -
Corbett Ronald J. T.,
Laptook Abbot R.,
Hassan A.,
Nunnally R. L.
Publication year - 1988
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910060112
Subject(s) - hypercarbia , intracellular ph , acidosis , inorganic phosphate , chemistry , in vivo , brain tissue , intracellular , pi , nuclear magnetic resonance spectroscopy , phosphate , nuclear magnetic resonance , medicine , biochemistry , biology , stereochemistry , physics , microbiology and biotechnology
31 P NMR brain spectra were obtained from piglets over a range of mild hypocarbia to severe hypercarbia ( P a CO 2 25 to 198 mm Hg). The chemical shifts of the phosphoethanolamine and inorganic phosphate were used to calculate intracellular brain pH (pH et and pH pi , respectively). Both pH pi and pH et underwent parallel significant decreases during hypercarbia, corresponding to 51 and 53% pH regulation , respectively. We conclude that the chemical shift of the phosphomonoester peak in vivo can be used to measure decreases in intracellular pH in neonatal brain. © 1988 Academic Press, Inc.