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Mobile phospholipid signals in NMR spectra of cultured human adenocarcinoma cells
Author(s) -
Guidoni L.,
Mariutti G.,
Rampelli G. M.,
Rosi A.,
Viti V.
Publication year - 1987
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910050609
Subject(s) - nuclear magnetic resonance , phospholipid , chemistry , nmr spectra database , nuclear magnetic resonance spectroscopy , spectral line , proton nmr , physics , biochemistry , membrane , astronomy
An intense peak is visible in the phosphodiester region of the 31 P NMR spectra of human colon adenocarcinoma HCT‐8R cells. A signal at the same frequency, partially deriving from mobile phospholipids, is also observable in the spectra of total membranes and of perchloric acid extracts prepared from the same cells. The phosphodiester signals of glycerophosphorylcholine, glycerophosphorylethanolamine, and glycerophosphorylserine resonate at lower fields with respect to this broader peak and appear only in aged samples. Enzymatic treatments of the extracts would indicate that ribonucleic acid also contributes to the broad phosphodiester signal. Moreover, 1 H NMR spectra show the presence of lipid structures, characterized by high mobility, in the cell and in the membrane samples, similar to what was already observed in other tumor cells and tissues. © Academic Press, Inc.