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In vitro proton T 1 and T 2 studies on rat liver: Analysis of multiexponential relaxation processes
Author(s) -
Barthwal R.,
Höhnberlage M.,
Gersonde K.
Publication year - 1986
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910030607
Subject(s) - relaxation (psychology) , triolein , liver tissue , chemistry , proton , magnetic relaxation , in vitro , nuclear magnetic resonance , physics , biochemistry , endocrinology , medicine , enzyme , magnetization , quantum mechanics , magnetic field , lipase
Abstract At 37°C and 20 MHz, the T 1 and T 2 proton relaxation processes in intact rat liver tissue are multiexponential functions which in the majority of cases were decomposed into a major (α* ≈ 90%, T 1 *= 374 ms, T 2 *= 58 ms) and a minor (α** ≈ 10%, T 1 **= 130 ms, T 2 **= 181 ms) component. Both, T 1 and T 2 , are temperature‐dependent with a temperature shift of Δ T 1 = 1.5 ms/°C and Δ T 2 = 0.5 ms/°C, respectively. Storage of liver tissue at 4°C and 37°C led to remarkable changes of the T 1 and T 2 values. For T 2 these changes occurred after a shorter storage time than for T 1 , but they are more pronounced for T 1 , To avoid such influences the relaxation measurements were performed within one hour after excision of the tissue. Even at 4°C, long‐term storage (>3h) must be avoided. A method for the quantitative determination of the fat content in liver based on multiexponential analysis of the T 1 relaxation process was evaluated employing mixtures of triolein with liver homogenate. Triolein is a two‐component system with T 1 *= 144 ms (α* = 62%) and T 1 **= 355 ms (α** = 38%). Finally, liver‐specific protocol conditions were defined for in vitro relaxation studies. © 1986 Academic Press, Inc.