Premium
Murine in vivo L‐band ESR spin‐label oximetry with a loop‐gap resonator
Author(s) -
Subczynski Witold K.,
Lukiewicz S.,
Hyde James S.
Publication year - 1986
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910030510
Subject(s) - electron paramagnetic resonance , oxygen , chemistry , resonator , analytical chemistry (journal) , in vivo , nuclear magnetic resonance , capsule , spin probe , paramagnetism , ion , materials science , optoelectronics , condensed matter physics , chromatography , physics , microbiology and biotechnology , organic chemistry , botany , biology
Small mice (∼20 g) were anesthetized and placed in a loop‐gap resonator of diameter 25 mm resonating at 1.1 GHz. An oxygen‐permeable capsule containing 2.5 × 10 −2 M perdeutero 15 N TEMPONE (1‐oxyl‐2, 2, 6, 6‐tetramethyl‐4‐piperidone) in light paraffin oil was implanted in the peritoneal cavity. Electron spin resonance (ESR) spectra from the capsule are sensitive to the concentration of dissolved oxygen and calibration curves are given. The oxygen concentration was found to rise from a value close to zero when the animal breathes air to a level of 220 μ M when the animal breaths pure oxygen. It is speculated that this surprisingly high level is related to the effect of the anesthetic on the cardiovascular system. Encapsulation provides a barrier to spin‐label reductants and to paramagnetic metal ions that might confound the spin‐label oximetric measurements. © 1986 Academic Press, Inc.