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A perfusion technique for 13 C NMR studies of the metabolism of 13 C‐labeled substrates by mammalian cells
Author(s) -
Lyon Robbe C.,
Faustino Patrick J.,
Cohen Jack S.
Publication year - 1986
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910030503
Subject(s) - perfusion , metabolism , agarose , chemistry , biochemistry , medicine
We describe an improved perfusion system designed for NMR studies with 13 C‐labeled metabolites. Using this system we have monitored the uptake and metabolism of [ 13 C]acetate and [ 13 ]Clglucose in pooled human lymphocytes cast in an agarose gel thread. © 1986 Academic Press, Inc.