Synthesis of [guanidino‐ 13 C]creatine and measurement of the creatine phosphokinase reaction  in vitro  by  13 C NMR spectroscopy | Zendy

Han Chung H. | Zendy; Sillerud Laurel O. | Zendy

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Synthesis of [guanidino‐ 13 C]creatine and measurement of the creatine phosphokinase reaction in vitro by 13 C NMR spectroscopy

Author(s) -

Han Chung H.,

Sillerud Laurel O.

Publication year - 1986

Publication title -

magnetic resonance in medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.696

H-Index - 225

eISSN - 1522-2594

pISSN - 0740-3194

DOI - 10.1002/mrm.1910030415

Subject(s) - phosphocreatine , creatine , sarcosine , chemistry , creatine kinase , cyanamide , nuclear magnetic resonance spectroscopy , carbon 13 , carbon 13 nmr , nuclear magnetic resonance , stereochemistry , biochemistry , glycine , amino acid , medicine , energy metabolism , physics , quantum mechanics

[guanidino‐ 13 C]Creatine has been synthesized by reaction involving synthesis of [ 13 C]cyanamide followed by its condensation with sarcosine. The creatine guanidino carbon 13 C NMR frequency shifts upon the formation of phosphocreatine by 0.83 ppm with a splitting ( 2 J CP = 3.3 Hz) due to two‐bond phosphorus‐carbon coupling. The high 13 C enrichment (99%) at the guanidino carbon of creatine gave rise to an adequate signal‐to‐noise ratio for both creatine and phosphocreatine so that a study of the time course of the creatine phosphokinase reaction could be performed and provided the means for a determination of the K m (15.5 m M ) of the reaction. © 1986 Academic Press, Inc.

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