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Synthesis of [guanidino‐ 13 C]creatine and measurement of the creatine phosphokinase reaction in vitro by 13 C NMR spectroscopy
Author(s) -
Han Chung H.,
Sillerud Laurel O.
Publication year - 1986
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910030415
Subject(s) - phosphocreatine , creatine , sarcosine , chemistry , creatine kinase , cyanamide , nuclear magnetic resonance spectroscopy , carbon 13 , carbon 13 nmr , nuclear magnetic resonance , stereochemistry , biochemistry , glycine , amino acid , medicine , energy metabolism , physics , quantum mechanics
[guanidino‐ 13 C]Creatine has been synthesized by reaction involving synthesis of [ 13 C]cyanamide followed by its condensation with sarcosine. The creatine guanidino carbon 13 C NMR frequency shifts upon the formation of phosphocreatine by 0.83 ppm with a splitting ( 2 J CP = 3.3 Hz) due to two‐bond phosphorus‐carbon coupling. The high 13 C enrichment (99%) at the guanidino carbon of creatine gave rise to an adequate signal‐to‐noise ratio for both creatine and phosphocreatine so that a study of the time course of the creatine phosphokinase reaction could be performed and provided the means for a determination of the K m (15.5 m M ) of the reaction. © 1986 Academic Press, Inc.