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31 P NMR detection of mobile dog brain phospholipids
Author(s) -
Cerdan S.,
Subramanian V. Harihara,
Hilberman M.,
Cone J.,
Egan J.,
Chance B.,
Williamson J. R.
Publication year - 1986
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910030308
Subject(s) - phosphodiester bond , phosphomonoesters , chemistry , phosphocreatine , in vivo , phosphate , phosphorus 31 nmr spectroscopy , nuclear magnetic resonance spectroscopy , phosphorus , nuclear magnetic resonance , biochemistry , stereochemistry , inorganic phosphate , rna , biology , organic chemistry , physics , microbiology and biotechnology , endocrinology , gene , energy metabolism
Abstract The in vivo dog brain 31 P NMR spectrum has a large peak in the phosphodiester region accounting for more than 35% of the total observable phosphorus metabolites. It is possible to reduce the intensity of this peak by off‐resonance saturation. To characterize the nature of this peak, extracts of dog brain frozen in situ were analyzed by high resolution 31 P NMR. ATP, phosphocreatine, inorganic phosphate, and phosphomonoesters were recovered in appropriate amounts in the methanol: HCI extract. However, acid soluble phosphodiesters accounted for only 8% of the observable phosphorus. More NMR observable phosphodiesters were selectively recovered following CHCI 3 :methanol extraction of phospholipids. These results suggest that the in vivo phosphodiester resonance has substantial contributions from a fraction of mobile brain phospholipids. © 1986 Academic Press, Inc.