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In vivo evaluation of intracellular pH and high‐energy phosphate metabolities during regional myocardial ischemia in cats using 31 P nuclear magnetic resonance
Author(s) -
Stein Paul D.,
Goldstein Sidney,
Sabbah Hani N.,
Liu ZhiQuan,
Helpern Joseph A.,
Ewing James R.,
Lakier Jeffrey B.,
Chopp Michael,
Lapenna William F.,
Welch Kenneth M. A.
Publication year - 1986
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910030209
Subject(s) - creatine , ischemia , cats , intracellular ph , phosphocreatine , adenosine triphosphate , ligation , high energy phosphate , medicine , phosphate , chemistry , creatine kinase , nuclear magnetic resonance spectroscopy , cardiology , intracellular , nuclear magnetic resonance , energy metabolism , biochemistry , physics , organic chemistry
Phosphorus‐31 nuclear magnetic resonance spectroscopy ( 31 P NMR) was used to asses the temporal changes of high‐energy phosphate metabolites in the region of acute myocardial ischemia of open‐chest cats. Eight anesthetized cats were studied following ligation of the left anterior descending coronary artery. Creatine phosphate showed a 79 ± 16% (mean ± SD) reduction by 4 min after the onset of ischemia. Prominent qualitative reductions of the spectral peak of creatine phosphate occurred by 40 s after ischemia. Adenosine triphosphate measured under the beta spectral peak (β‐ATP) decreased 37 ± 9% by 20–25 min after ligation of the left anterior descending coronary artery. These reductions developed more slowly and were of smaller magnitude than those of creatine phosphate. Intracellular pH decreased from 7.39 ± 0.07 to 7.13 ± 0.09 units by 40 s after ischemia. By 30 min, pH decreased to 6.07 ± 0.40 units. The study shows, therefore, the temporal changes of high‐energy phosphate metabolites during ischemia in localized regions of the myocardium of open‐chest animals. © 1986 Academic Press, Inc.