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31 P Surface‐Coil NMR Analysis of Metabolic Status in KHJJ Tumors
Author(s) -
Adams Dorothy A.,
Denardo Gerald L.,
Denardo Sally J.,
Conboy Claire B.,
Morton Bradbury E.
Publication year - 1985
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910020502
Subject(s) - radioimmunotherapy , nuclear magnetic resonance spectroscopy , chemistry , necrosis , monoclonal antibody , cancer research , antibody , nuclear magnetic resonance , pathology , medicine , immunology , physics , organic chemistry
Noninvasive monitoring of the effects of treatment on cancer tissue is fundamental to the development of rational radioimmunotherapy (RIT) schemes employing radiolabeled monoclonal antibodies or antibody fragments specific for human cancer. Recent advances in nuclear magnetic resonance spectroscopy make it an attractive candidate for sequential, topical monotoring by 31 P NMR of metabolic events during RIT. Preliminary to this effort, we examined the metabolic competence of the well‐characterized, murine KHJJ tumor in situ in BALB/c mice using 31 P surface‐coil NMR. The ATP/ P i ratio in tumor volumes ranging from 100 to 800 mm 3 showed that tumors over this range of sizes were able to maintain high levels of ATP relative to P i . As the tumor volume increased above 1 cm 3 , ATP/ P i levels indicated poor metabolic competence. This lack of metabolic competence was correlated with histological evidence of tissue necrosis and vascular disintegration. The T 1 values of assigned phosphorus metabolites were established. Intracellular pH, as determined from the chemical shift of P i , was found to vary in these tumors from 7.1 in rapidly metabolizing tissue to 6.6 in necrotic tumors.© 1985 Academic Press,Inc.