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A versatile perfusion technique for metabolic studies by NMR
Author(s) -
Geoffrion Yves,
Lareau Sylvain,
Deslauriers Roxanne,
Butler Keith,
Pass Michael,
Smith Ian C. P.
Publication year - 1985
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910020108
Subject(s) - lipogenesis , gluconeogenesis , perfusion , chemistry , agarose , biochemistry , metabolism , medicine
A perfusion technique applicable to metabolic studies by NMR is described in detail. The utility and versatility of the approach are demonstrated by following gluconeogenesis from [2‐ 13 C]pyruvate in the perfused, isolated mouse liver, and lipogenesis from [2‐ 13 C]acetate in perfused Acanthamoeba castellanii cells embedded in agarose filaments.