Premium
Application of multipulse NMR to observe 13 C‐labeled metabolites in biological systems
Author(s) -
Bendall M. Robin,
Hollander Jan A. Den,
AriasMendoza Fernando,
Rothman Douglas L.,
Behar Kevin L.,
Shulman Robert G.
Publication year - 1985
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1910020107
Subject(s) - pulse sequence , dept , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , sequence (biology) , chemistry , nmr spectra database , in vivo , spin echo , resolution (logic) , analytical chemistry (journal) , spectral line , physics , magnetic resonance imaging , computer science , chromatography , biology , stereochemistry , biochemistry , artificial intelligence , medicine , microbiology and biotechnology , radiology , astronomy
Limitations in resolution and sensitivity of 13 C NMR spectroscopy have reduced the information obtainable from intact biological systems. With the aim of increasing the information from in vivo 13 C NMR two multipulse NMR techniques, the DEPT pulse sequence and the gated spin‐echo sequence, were used to obtain edited 13 C NMR spectra from different 13 C‐labeled mammalian tissues. This allowed the separation of the 13 C NMR signals from the tissues into subspectra containing either CH, CH 2 , or CH 3 signals, thereby increasing the information obtainable from these spectra. Comparing the two techniques, the DEPT sequence gives more accurate editing than the gated spin‐echo sequence but suffers from the difficulty of determining 1 H pulse angles in vivo.