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Application of 19 F chemical shift imaging in studies of mice with orally administered 5‐fluorouracil
Author(s) -
Kuribayashi Hideto,
Doi Yoshihiro,
Kanazawa Yoko
Publication year - 2001
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1270
Subject(s) - chemistry , sequence (biology) , small intestine , nuclear magnetic resonance , sampling (signal processing) , signal (programming language) , in vivo , resolution (logic) , stomach , nuclear medicine , analytical chemistry (journal) , physics , chromatography , medicine , optics , biology , biochemistry , detector , computer science , microbiology and biotechnology , artificial intelligence , programming language
In vivo quantitative metabolic mapping is an ideal tool for pharmacokinetic studies. Oral 5‐fluorouracil (5‐FU) and its metabolites in mice were imaged simultaneously by the 19 F fast spin echo (FSE) sequence using interleaved frequency selection at 9.4T. However, 5‐FU images in the small intestine have never been obtained regardless of concentration. The reason for the discrepancy between image intensity and concentration was T 2 . At a pH above 6, a dramatic decrease in T 2 of a 19 F 5‐FU signal in an aqueous solution was found; T 2 was shorter in the small intestine (14 ms) than in the stomach (52 ms). The 19 F CSI sequence in FID sampling mode was employed for detecting short T 2 signals. With a 13‐min resolution time, the detection of the 5‐FU signals in the region of the small intestine (0.6 mmol/kg) was successful with a 5 × 5 mm 2 in‐plane resolution. Furthermore, two signals separated by 2 ppm were clearly distinguishable, but failed to be separately detectable with the 19 F FSE sequence. For quantitative simultaneous monitoring of 5‐FU and its metabolites of varying T 2 , the 19 F CSI sequence in FID sampling mode was found to be superior to the 19 F FSE sequence. Magn Reson Med 46:864–869, 2001. © 2001 Wiley‐Liss, Inc.