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A serial MR study of cerebral blood flow changes and lesion development following endothelin‐1‐induced ischemia in rats
Author(s) -
Biernaskie Jeff,
Corbett Dale,
Peeling James,
Wells Jennifer,
Lei Hao
Publication year - 2001
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1263
Subject(s) - lesion , cerebral blood flow , ischemia , middle cerebral artery , medicine , perfusion , endothelin 1 , blood flow , striatum , stroke (engine) , brain ischemia , pathology , anesthesia , cardiology , dopamine , mechanical engineering , receptor , engineering
Abstract The vasoconstrictive peptide endothelin‐1 (ET‐1) has been used previously to transiently occlude the middle cerebral artery (MCA) in rats. However, the duration of the resulting reduction in cerebral blood flow (CBF) and the reperfusion characteristics are poorly understood. In this study perfusion and T 2 ‐weighted MRI were used together with histology to characterize the cerebral perfusion dynamics and lesion development following ET‐1 injection. Twenty‐two rats received an intracerebral injection of ET‐1 adjacent to the MCA. CBF was reduced to 30–50% of control levels, and a significant reduction persisted for 16 h in the cortex and 7 h in the striatum. The lesion size measured by T 2 ‐weighted imaging at 48 h correlated with the final infarct size measured by histology at 7 d. The sustained reduction in CBF and the gradual development of the ischemic lesion resemble human stroke evolution, suggesting that this model may be useful for evaluating therapeutic agents, particularly when treatment is delayed. Magn Reson Med 46:827–830, 2001. © 2001 Wiley‐Liss, Inc.