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MR microscopy of cobalt‐labeled nerve cells and pathways in an invertebrate brain ( Sepia officinalis , Cephalopoda)
Author(s) -
Quast Michael J.,
Neumeister Heike,
Ezell Edward L.,
Budelmann Bernd U.
Publication year - 2001
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.1078
Subject(s) - sepia , cuttlefish , cobalt , nerve cells , anatomy , officinalis , iontophoresis , biology , chemistry , biophysics , neuroscience , microbiology and biotechnology , paleontology , botany , inorganic chemistry
This article describes a novel application of contrast‐enhanced MR microscopy to trace nerve cells and pathways through small invertebrate brains. Using the cuttlefish Sepia officinalis (Cephalopoda) as a model, the cells and pathways of one of the brain nerves were labeled with paramagnetic cobalt(II) ions by conventional centripetal cobalt iontophoresis. In MR microscopy, the cobalt‐labeled cell bodies and pathways became hypointense in 9.4 T spin echo images. Their course and distribution were identical with those seen with conventional histological techniques after cobalt sulphide precipitation (with or without subsequent silver intensification). Magn Reson Med 45:575–579, 2001. © 2001 Wiley‐Liss, Inc.