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1 H NMR detection of vitamin C in human brain in vivo
Author(s) -
Terpstra Melissa,
Gruetter Rolf
Publication year - 2004
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.10715
Subject(s) - in vivo , chemistry , phosphocholine , human brain , ascorbic acid , vitamin c , nuclear magnetic resonance spectroscopy , vitamin , biochemistry , nuclear magnetic resonance , biology , phospholipid , stereochemistry , phosphatidylcholine , microbiology and biotechnology , food science , neuroscience , membrane , physics
Vitamin C (ascorbate) is well established as an essential nutrient that functions as an antioxidant. Since it is present in the human brain at detectable concentrations, this study was designed to detect and quantify ascorbate in the human brain in vivo using 1 H NMR spectroscopy (MRS). Ascorbate was consistently detected in all five study subjects, and was measured using MEGA‐PRESS difference editing. The in vivo resonance pattern was consistent with that of ascorbate based on position, line width, peak pattern, and relative intensity. Metabolites with a potential for coediting were assessed using phantom solutions. The putative resonances of myo‐inositol, lactate, glycerophosphocholine, phosphocholine, and phosphoethanolamine were detected at positions distinct from those of ascorbate. This study represents the first in vivo detection of vitamin C in the human brain using 1 H MRS. A concentration of 1.3 ± 0.3 μmol/g (mean ± SD, N = 4) was estimated. Magn Reson Med 51:225–229, 2004. © 2004 Wiley‐Liss, Inc.