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Quantitation of differential renal blood flow and renal function using dynamic contrast‐enhanced MRI in rats
Author(s) -
Pedersen Michael,
Shi Yimin,
Anderson Peter,
StødkildeJørgensen Hans,
Christian Djurhuus Jens,
Gordon Isky,
Frøkiær Jørgen
Publication year - 2004
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.10711
Subject(s) - renal blood flow , renal function , kidney , chemistry , blood flow , renal artery , bolus (digestion) , gadolinium , occlusion , nuclear medicine , intensity (physics) , urology , medicine , nuclear magnetic resonance , physics , organic chemistry , quantum mechanics
Abstract A first‐pass upslope approach was used to estimate differential renal blood flow (DRBF) and a Patlak‐Rutland approach was used to estimate differential renal function (DRF) using Gd‐DTPA‐BMA‐enhanced MRI. DRBF and DRF were estimated in rat kidneys under three different experimental conditions: 1) transient renal artery occlusion (TRAO); 2) partial unilateral ureteric obstruction (PUO); and 3) sham‐operated control rats (SHAM). A bolus of Gd‐DTPA‐BMA was given intravenously during a dynamic single slice T 1 ‐weighted gradient echo sequence, which allowed calculation of concentration from signal intensity values. Calculations based on the raw signal intensity showed that DRBF was decreased in both PUO (44 ± 1%; P < 0.05) and in TRAO (38 ± 1%; P < 0.05) compared with SHAM (52 ± 1%). Converting the signal intensity into a measure of Gd‐DTPA‐BMA concentration did not substantially alter these findings (PUO: 40 ± 3%, P < 0.05; TRAO: 35 ± 2%, P < 0.05; SHAM 49 ± 1%). Likewise, DRF decreased in both PUO (43 ± 4%; P < 0.05) and TRAO (39 ± 3%; P < 0.05) compared with SHAM (48 ± 2%). Converting the signal intensity into measurements of Gd‐DTPA‐BMA concentration revealed similar findings (PUO: 41 ± 5%, P < 0.05; TRAO: 34 ± 5%, P < 0.05; SHAM: 49 ± 2%). Our results suggest that renal damage in rats may be demonstrated by an observed reduction of DRBF and DRF as estimated from single‐slice Gd‐DTPA‐BMA enhanced signal intensity using time–activity curves with and without quantitation of Gd‐concentration. Magn Reson Med 51:510–517, 2004. © 2004 Wiley‐Liss, Inc.