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Proton HR‐MAS spectroscopy and quantitative pathologic analysis of MRI/3D‐MRSI‐targeted postsurgical prostate tissues
Author(s) -
Swanson Mark G.,
Vigneron Daniel B.,
Tabatabai Z. Laura,
Males Ryan G.,
Schmitt Lars,
Carroll Peter R.,
James Joyce K.,
Hurd Ralph E.,
Kurhanewicz John
Publication year - 2003
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.10614
Subject(s) - prostate cancer , phosphocholine , prostate , choline , in vivo magnetic resonance spectroscopy , magnetic resonance spectroscopic imaging , stromal cell , in vivo , chemistry , pathology , cancer , medicine , magnetic resonance imaging , phospholipid , biology , biochemistry , radiology , microbiology and biotechnology , membrane , phosphatidylcholine
Proton high‐resolution magic angle spinning ( 1 H HR‐MAS) NMR spectroscopy and quantitative histopathology were performed on the same 54 MRI/3D‐MRSI‐targeted postsurgical prostate tissue samples. Presurgical MRI/3D‐MRSI targeted healthy and malignant prostate tissues with an accuracy of 81%. Even in the presence of substantial tissue heterogeneity, distinct 1 H HR‐MAS spectral patterns were observed for different benign tissue types and prostate cancer. Specifically, healthy glandular tissue was discriminated from prostate cancer based on significantly higher levels of citrate ( P = 0.04) and polyamines ( P = 0.01), and lower ( P = 0.02) levels of the choline‐containing compounds choline, phosphocholine (PC), and glycerophosphocholine (GPC). Predominantly stromal tissue lacked both citrate and polyamines, but demonstrated significantly ( P = 0.01) lower levels of choline compounds than cancer. In addition, taurine, myo‐inositol, and scyllo‐inositol were all higher in prostate cancer vs. healthy glandular and stromal tissues. Among cancer samples, larger increases in choline, and decreases in citrate and polyamines ( P = 0.05) were observed with more aggressive cancers, and a MIB‐1 labeling index correlated (r = 0.62, P = 0.01) with elevated choline. The elucidation of spectral patterns associated with mixtures of different prostate tissue types and cancer grades, and the inclusion of new metabolic markers for prostate cancer may significantly improve the clinical interpretation of in vivo prostate MRSI data. Magn Reson Med 50:944–954, 2003. © 2003 Wiley‐Liss, Inc.