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1 H‐localized broadband 13 C NMR spectroscopy of the rat brain in vivo at 9.4 T
Author(s) -
Henry PierreGilles,
Tkáč Ivan,
Gruetter Rolf
Publication year - 2003
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.10601
Subject(s) - in vivo , glutamine , nuclear magnetic resonance , glutamate receptor , chemistry , nuclear magnetic resonance spectroscopy , alanine , amino acid , biochemistry , stereochemistry , biology , physics , receptor , microbiology and biotechnology
Localized 13 C NMR spectra were obtained from the rat brain in vivo over a broad spectral range (15–100 ppm) with minimal chemical‐shift displacement error (<10%) using semi‐adiabatic distortionless enhancement by polarization transfer (DEPT) combined with 1 H localization. A new gradient dephasing scheme was employed to eliminate unwanted coherences generated by DEPT when using surface coils with highly inhomogeneous B 1 fields. Excellent sensitivity was evident from the simultaneous detection of natural abundance signals for N‐acetylaspartate, myo ‐inositol, and glutamate in the rat brain in vivo at 9.4 T. After infusion of 13 C‐labeled glucose, up to 18 13 C resonances were simultaneously measured in the rat brain, including glutamate C2, C3, C4, glutamine C2, C3, C4, aspartate C2, C3, glucose C1, C6, N‐acetyl‐aspartate C2, C3, C6, as well as GABA C2, lactate C3, and alanine C3. 13 C‐ 13 C multiplets corresponding to multiply labeled compounds were clearly observed, suggesting that extensive isotopomer analysis is possible in vivo. This unprecedented amount of information will be useful for metabolic modeling studies aimed at understanding brain energy metabolism and neurotransmission in the rodent brain. Magn Reson Med 50:684–692, 2003. © 2003 Wiley‐Liss, Inc.