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Improved molecular imaging contrast agent for detection of human thrombus
Author(s) -
Winter Patrick M.,
Caruthers Shelton D.,
Yu Xin,
Song ShengKwei,
Chen Junjie,
Miller Brad,
Bulte Jeff W.M.,
Robertson J. David,
Gaffney Patrick J.,
Wickline Samuel A.,
Lanza Gregory M.
Publication year - 2003
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.10532
Subject(s) - thrombus , fibrin , gadolinium , molecular imaging , chemistry , molecular mass , magnetic resonance imaging , mri contrast agent , nuclear magnetic resonance , biophysics , medicine , radiology , biochemistry , cardiology , in vivo , immunology , physics , microbiology and biotechnology , organic chemistry , biology , enzyme
Molecular imaging of microthrombus within fissures of unstable atherosclerotic plaques requires sensitive detection with a thrombus‐specific agent. Effective molecular imaging has been previously demonstrated with fibrin‐targeted Gd‐DTPA‐bis‐oleate (BOA) nanoparticles. In this study, the relaxivity of an improved fibrin‐targeted paramagnetic formulation, Gd‐DTPA‐phosphatidylethanolamine (PE), was compared with Gd‐DTPA‐BOA at 0.05‐4.7 T. Ion‐ and particle‐based r 1 relaxivities (1.5 T) for Gd‐DTPA‐PE (33.7 (s*mM) ‐1 and 2.48 × 10 6 (s*mM) ‐1 , respectively) were about twofold higher than for Gd‐DTPA‐BOA, perhaps due to faster water exchange with surface gadolinium. Gd‐DTPA‐PE nanoparticles bound to thrombus surfaces via anti‐fibrin antibodies (1H10) induced 72% ± 5% higher change in R 1 values at 1.5 T (Δ R 1 = 0.77 ± 0.02 1/s) relative to Gd‐DTPA‐BOA (Δ R 1 = 0.45 ± 0.02 1/s). These studies demonstrate marked improvement in a fibrin‐specific molecular imaging agent that might allow sensitive, early detection of vascular microthrombi, the antecedent to stroke and heart attack. Magn Reson Med 50:411–416, 2003. © 2003 Wiley‐Liss, Inc.