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Determinants of intramyocellular lipid concentrations in rat hindleg muscle
Author(s) -
NeumannHaefelin Claudia,
Kuhlmann Johanna,
Belz Ulrich,
Kalisch Juergen,
Quint Manfred,
Gerl Martin,
Juretschke HansPaul,
Herling Andreas W.
Publication year - 2003
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.10518
Subject(s) - medicine , endocrinology , insulin resistance , type 2 diabetes , insulin , biology , diabetes mellitus
The investigation of intramyocellular lipids (IMCLs) with proton MR spectroscopy ( 1 H‐MRS) in humans has recently received increasing attention. IMCL levels correlate with insulin resistance and are affected by diet and exercise, making IMCL an interesting marker for metabolic investigations. In the present in vivo study, the feasibility of using 1 H MRS for the detection of IMCL in rats is demonstrated, and the influence of various factors, such as age, gender, muscle type, and rat strain, on IMCL levels is systematically analyzed. In healthy Wistar and Sprague Dawley (SD) rats, the highest ratios of IMCL/tCr were found in young rats, and IMCL/tCr decreased with increasing age. In addition, IMCL concentration was clearly influenced by gender and muscle type. Insulin‐resistant, male, obese, Zucker diabetic fatty (ZDF) rats showed significantly higher IMCL levels than Wistar or SD rats. In conclusion, although IMCL levels are clearly influenced by insulin resistance, several other factors influence IMCL levels, such as age, gender, muscle type, and rat strain. Therefore, when using IMCL as a surrogate marker for insulin resistance, it is necessary to carefully compare results with age‐ and gender‐matched controls, and to use identical conditions. Magn Reson Med 50:242–248, 2003. © 2003 Wiley‐Liss, Inc.