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Effect of nicotinamide supplementation in in vitro fertilization medium on bovine embryo development
Author(s) -
Yuan YuGuo,
Mesalam Ayman,
Song SeokHwan,
Lee KyeongLim,
Xu Lianguang,
Joo MyeongDon,
Kong IlKeun
Publication year - 2020
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.23417
Subject(s) - biology , embryo , blastocyst , andrology , reactive oxygen species , embryogenesis , human fertilization , oxidative stress , nicotinamide , in vitro , in vitro fertilisation , apoptosis , microbiology and biotechnology , biochemistry , anatomy , medicine , enzyme
Increased oxidative stress is one of the main causes of poorly developed embryos in assisted reproductive technologies. Nicotinamide (NAM) has been shown to suppress reactive oxygen species (ROS) production through its potent antioxidative and anti‐senescent effects. In the present study, we explored the effects of short‐term NAM‐treatment (3 and 5 h) during in vitro fertilization (IVF) on the development of bovine embryos. Treatment with 10 mM NAM for 3 h significantly increased the blastocyst formation but extending the treatment to 5 h did not enhance the benefits any further. Immunofluorescence analysis demonstrated that treatment with 10 mM NAM for 3 h decreased the expression of intracellular ROS, 8‐oxo‐7,8‐dihydroguanine, caspase‐3, and increased the expression of Sirt1, and incorporation of bromodeoxyuridine in one‐cell stage embryos. Similarly, the level of H3K56ac significantly increased in the NAM‐treated (3 and 5 h) one‐cell stage embryos. Contrastingly, the treatment with 10 mM NAM for 5 h increased the caspase‐9 level in blastocysts. Collectively, these findings suggest that NAM possesses antioxidant activity and supplementation of IVF medium with 10 mM NAM for 3 h improves the in vitro developmental competence of bovine embryos.