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Generation of Footprint‐Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector
Author(s) -
Tsukamoto Masaya,
Kimura Kazuto,
Tanaka Miyuu,
Kuwamura Mitsuru,
Ohtaka Manami,
Nakanishi Mahito,
Sugiura Kikuya,
Hatoya Shingo
Publication year - 2020
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.23349
Subject(s) - sendai virus , biology , peripheral blood mononuclear cell , induced pluripotent stem cell , virology , germ layer , virus , cell culture , vector (molecular biology) , viral vector , microbiology and biotechnology , in vitro , genetics , recombinant dna , gene , embryonic stem cell
Using auto‐erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT‐PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one of the good cell sources to generate ciPSC lines from companion and patient dogs.