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Differential gene regulation in selected lines for high and low sperm production in male guppies
Author(s) -
Cattelan Silvia,
Vidotto Michele,
Devigili Alessandro,
Pilastro Andrea,
Grapputo Alessandro
Publication year - 2020
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.23332
Subject(s) - biology , sperm , transcriptome , sperm competition , spermatogenesis , human fertilization , genetics , gene , andrology , embryo , gene expression , evolutionary biology , endocrinology , medicine
Abstract In species where females mate with more than one male during the same reproductive event, males typically increase the number of sperm produced to boost their fertilization share. Sperm is not limitless, however, and theory predicts that their production will come at the cost of other fitness‐related traits, such as body growth or immunocompetence, although these evolutionary trade‐offs are notoriously difficult to highlight. To this end, we combined artificial selection for sperm production with a transcriptome analysis using Poecilia reticulata , a fish characterized by intense sperm competition in which the number of sperm transferred during mating is the most important predictor of fertilization success, yet sperm production is highly variable among males. We compared the brain and testes transcriptome in male guppies of lines artificially selected for high and low sperm production by identifying pivotal differentially expressed gene sets that may regulate spermatogenesis and immune function in this species. Despite the small differences in single genes' expression, gene set enrichment analysis showed coordinated gene expression differences associated with several pathways differentially regulated in the two selection lines. High sperm production males showed an upregulation of pathways related to immunosuppression and development of spermatozoa indicating a possible immunological cost of sperm production.