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Birth of a marmoset following injection of elongated spermatid from a prepubertal male
Author(s) -
Ogonuki Narumi,
Abe Yukiko,
Kurotaki Yoko K.,
Nakao Kazuki,
Aiba Atsu,
Sasaki Erika,
Ogura Atsuo
Publication year - 2019
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.23220
Subject(s) - spermatid , marmoset , biology , andrology , sperm , offspring , embryo , gamete , spermatogenesis , spermiogenesis , transgenesis , mating , physiology , genetics , embryogenesis , reproductive biology , endocrinology , pregnancy , medicine , paleontology
The common marmoset is a small nonhuman primate in which the application of transgenesis and genetic knockout techniques allows the generation of gene‐modified models of human diseases. However, its longer generation time than that of rodents is a major obstacle to the widespread use of gene‐modified marmosets for biomedical research. In this study, we examined the feasibility of shortening the generation time by using prepubertal marmoset males as gamete donors. We collected late round stage spermatids (Steps 5–7), elongated spermatids, and testicular spermatozoa from the testis of a prepubertal 11‐month‐old male marmoset and injected them into in vitro‐matured oocytes. After 7 days in culture, two embryos from elongated spermatid injection and two embryos from sperm injection were transferred into two separate recipient females. The recipient female that received elongated spermatid injection‐derived embryos became pregnant and gave birth to one female infant. This is the first demonstration that a spermatid from a prepubertal male primate can support full‐term development. Using this method, we can expect to obtain offspring of gene‐modified males 6 months to a year earlier than with natural mating