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Lipidomic profile as a noninvasive tool to predict endometrial receptivity
Author(s) -
Braga Daniela Paes de Almeida Ferreira,
Borges Edson,
Godoy Adriana Teixeira,
Montani Daniela Antunes,
Setti Amanda Souza,
Zanetti Bianca Ferrarini,
Figueira Rita de Cássia Sávio,
Eberlin Marcos Nogueira,
Lo Turco Edson Guimarães
Publication year - 2019
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.23088
Subject(s) - receiver operating characteristic , principal component analysis , biology , area under the curve , biomarker , lipidomics , medicine , mathematics , bioinformatics , statistics , biochemistry
For the present study we asked whether the endometrial fluid lipidomic may be a useful approach to predict endometrial receptivity in freeze‐all cycles. For this case‐control study, endometrial fluid samples were collected from 41 patients undergoing freeze‐all cycles. Samples were split depending on the pregnancy outcome: positive group ( n = 24) and negative group ( n = 17). Data were acquired by the matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS‐DA) were applied. A list of potential biomarker ion ratios was obtained and the values were used to build a receiver operating characteristic (ROC) curve to predict pregnancy success. The lipid categories were attributed by LIPID MAPS database. Ion ratios were established according to their correlations and used for the analysis. The PCA showed a tendency of separation between the studied groups, whereas the PLS‐DA was able to clearly distinguish them. Fifteen ratios (13 hyper‐represented in the negative and two hyper‐represented in the positive group) were selected according to their importance for model prediction. These ratios were used to build the ROC curve, which presented an area under curve of 84.0% (95%CI: 69.2–97.4%; p = 0.009). These findings suggest that lipidomic profiling of endometrial fluid may be a valuable tool for identifying the time interval comprising the window of implantation.