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The role of Melatonin receptor MTNR1A in the action of Melatonin on bovine granulosa cells
Author(s) -
Wang ShuJuan,
Liu WenJu,
Wang LiKe,
Pang XunSheng,
Yang LiGuo
Publication year - 2017
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22877
Subject(s) - melatonin , biology , medicine , endocrinology , melatonin receptor , gene knockdown , follistatin , inha , follicular atresia , cholesterol side chain cleavage enzyme , ovary , ovarian follicle , apoptosis , metabolism , cytochrome p450 , biochemistry , tuberculosis , pathology , isoniazid
Granulosa cells (GCs) play an important role in ovarian follicle growth, development, and follicular atresia. In the present study, we investigated the effects of Melatonin on bovine GCs, and asked if MTNR1A was involved in their response to this indole hormone. Our results indicated that Melatonin inhibited GC apoptosis by up‐regulating the expression of BCL2 , BCL‐XL , GPX4 , and SOD1 , and down‐regulating the expression of BAX , CASP3 , and TP53 . Moreover, Melatonin modulated bovine GC function by decreasing the expression of INHA , INHBB , FSHR , and TGFBR3 , and the abundance of Inhibin β and Activin B, while increasing the expression of LHR , INHBA , and secretion of progesterone by GCs. In contrast, knockdown of MTNR1A significantly increased the expression of BAX , CASP3 , TP53 , INHA , FSHR , and TGFBR3 , as well as Inhibin β abundance, while decreasing the expression of BCL2 , GPX4 , SOD1 , and LHR , and production of progesterone and estradiol; no effect was observed on the expression of BCL‐XL , INHBA , or INHBB . These results suggest that Melatonin and MTNR1A play an important role in modulating bovine GC function by regulating cellular progression, apoptosis, hormones secretion, and reproduction‐related genes. Furthermore, altered expression of MTNR1A could affect how bovine GCs respond to Melatonin.