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Developmental competence of porcine genome‐edited zygotes
Author(s) -
Gil Maria A.,
Martinez Cristina A.,
Nohalez Alicia,
Parrilla Inmaculada,
Roca Jordi,
Wu Jun,
Ross Pablo J.,
Cuello Cristina,
Izpisua Juan C.,
Martinez Emilio A.
Publication year - 2017
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22829
Subject(s) - biology , genome editing , transcription activator like effector nuclease , zygote , genome , biomedicine , crispr , cas9 , microinjection , genetics , computational biology , zinc finger nuclease , embryo , gene , microbiology and biotechnology , embryogenesis
Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site‐specific nucleases—including ZFNs, TALENs, and the CRISPR/Cas9 system—has popularized targeted zygote genome editing via one‐step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome‐edited porcine embryos and strategies to improve the zygote genome‐editing efficiency in pigs.