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Quisinostat treatment improves histone acetylation and developmental competence of porcine somatic cell nuclear transfer embryos
Author(s) -
Jin Long,
Guo Qing,
Zhu HaiYing,
Xing XiaoXu,
Zhang GuangLei,
Xuan MeiFu,
Luo QiRong,
Luo ZhaoBo,
Wang JunXia,
Yin XiJun,
Kang JinDan
Publication year - 2017
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22787
Subject(s) - somatic cell nuclear transfer , biology , blastocyst , embryo , epigenetics , andrology , acetylation , reprogramming , somatic cell , histone , histone deacetylase inhibitor , embryogenesis , histone deacetylase , microbiology and biotechnology , genetics , gene , medicine
Abnormal epigenetic modifications are considered a main contributing factor to low cloning efficiency. In the present study, we explored the effects of quisinostat, a novel histone deacetylase inhibitor, on blastocyst formation rate in porcine somatic‐cell nuclear transfer (SCNT) embryos, on acetylation of histone H3 lysine 9 (AcH3K9), and on expression of POU5F1 protein and apoptosis‐related genes BAX and BCL2 . Our results showed that treatment with 10 nM quisinostat for 24 hr significantly improved the development of reconstructed embryos compared to the untreated group (19.0 ± 1.6% vs. 10.2 ± 0.9%; p < 0.05). Quisinostat‐treated SCNT embryos also possessed significantly increased AcH3K9 at the pseudo‐pronuclear stage ( p < 0.05), as well as improved immunostaining intensity for POU5F1 at the blastocyst stage ( p < 0.05). While no statistical difference in BAX expression was observed, BCL2 transcript abundance was significantly different in the quisinostat‐treated compared to the untreated control group. Of the 457 quisinostat‐treated cloned embryos transferred into three surrogates, six fetuses developed from the one sow that became pregnant. These findings suggested that quisinostat can regulate gene expression and epigenetic modification, facilitating nuclear reprogramming, and subsequently improving the developmental competence of pig SCNT embryos and blastocyst quality.