Premium
The mu (μ) and delta (δ) opioid receptors modulate boar sperm motility
Author(s) -
VicenteCarrillo Alejandro,
ÁlvarezRodríguez Manuel,
RodríguezMartínez Heriberto
Publication year - 2016
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22675
Subject(s) - biology , motility , receptor , boar , sperm motility , delta , opioid , δ opioid receptor , sperm , microbiology and biotechnology , genetics , aerospace engineering , engineering
SUMMARY Endogenous and exogenous opioids modulate reproductive functions in target cells via opioid receptors (μ, δ, and κ). Sperm motility is a metric of gamete functionality, and serves as a suitable parameter for in vitro drug‐induced toxicity assays. This study identifies the presence and location of opioid receptors in pig spermatozoa as well as their functional response after in vitro challenge with known agonists (morphine [μ]; [D‐Pen 2,5]‐enkephanile [δ]; and U 50488 [κ]) and antagonists (naloxone [μ]; naltrindole [δ]; and nor ‐binaltrorphimine [κ]). Only the μ‐ and δ‐opioid receptors were present in the boar sperm plasma membrane, overlying the acrosome, neck, and principal piece. Challenge experiments with agonists and antagonists identified both μ‐ and δ‐opioid receptors as regulators of sperm kinematics, wherein μ maintains or increases sperm movement whereas δ decreases sperm motility over time. Mol. Reprod. Dev. 83: 724–734, 2016 © 2016 Wiley Periodicals, Inc .