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A novel TRIM family member, Trim69, regulates zebrafish development through p53‐mediated apoptosis
Author(s) -
Han Ruiqin,
Zhao Qing,
Zong Shudong,
Miao Shiying,
Song Wei,
Wang Linfang
Publication year - 2016
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22643
Subject(s) - zebrafish , biology , gene knockdown , apoptosis , microbiology and biotechnology , phenotype , genetics , gene
SUMMARY Trim69 contains the hallmark domains of a tripartite motif (TRIM) protein, including a Ring‐finger domain, B‐box domain, and coiled‐coil domain. Trim69 is structurally and evolutionarily conserved in zebrafish, mouse, rat, human, and chimpanzee. The role of this protein is unclear, however, so we investigated its function in zebrafish development. Trim69 is extensively expressed in zebrafish adults and developing embryos—particularly in the testis, brain, ovary, and heart—and its expression decreases in a time‐ and stage‐dependent manner. Loss of trim69 in zebrafish induces apoptosis and activates apoptosis‐related processes; indeed, the tp53 pathway was up‐regulated in response to the knockdown. Expression of human trim69 rescued the apoptotic phenotype, while overexpression of trim69 does not increase cellular apoptosis. Taken together, our results suggest that trim69 participates in tp53 ‐mediated apoptosis during zebrafish development. Mol. Reprod. Dev. 83: 442–454, 2016. © 2016 Wiley Periodicals, Inc .