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Effects of Ubiquitin C‐Terminal Hydrolase L1 (UCH‐L1) inhibition on sperm incorporation and cortical tension in mouse eggs
Author(s) -
Christianson Mindy S.,
Gerolstein Amanda L.,
Lee Hyo J.,
Monseur Brent C.,
Robinson Douglas N.,
Evans Janice P.
Publication year - 2016
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22617
Subject(s) - public health , reproductive endocrinology , biology , library science , medicine , endocrinology , hormone , pathology , computer science
Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) is thought to have multiple functions in mammalian oocytes and early embryos (e.g., Mtango et al. 2011; Sekiguchi et al. 2006); indeed, UCH-L1 is enriched in the egg cortex (Sekiguchi et al. 2006). Here we asked what UCH-L1 does at the mouse egg cortex and/or overlying plasma membrane. We examined egg membrane receptivity to sperm by assessing sperm incorporation over time with zona pellucida-free eggs, as previously described (McAvey et al. 2002), in the presence or absence of the UCH-L1 inhibitor LDN-57444, the development and specificity of which was described by Liu et al. (2003). This article is protected by copyright. All rights reserved.