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Testicular lactate content is compromised in men with Klinefelter Syndrome
Author(s) -
Alves Marco G.,
Martins Ana D.,
Jarak Ivana,
Barros Alberto,
Silva Joaquina,
Sousa Mário,
Oliveira Pedro F.
Publication year - 2016
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22608
Subject(s) - biology , glut3 , klinefelter syndrome , endocrinology , spermatogenesis , andrology , male infertility , azoospermia , glut1 , medicine , lactate dehydrogenase , glycolysis , cyp17a1 , lactate dehydrogenase a , infertility , glucose transporter , genetics , metabolism , insulin , enzyme , biochemistry , gene , pregnancy
SUMMARY Klinefelter syndrome (KS) is the most common genetic cause of human infertility, but the mechanism(s) responsible for its phenotype remain largely unknown. KS is associated with alterations in body composition and with a higher risk of developing metabolic diseases. We therefore hypothesized that KS men seeking fertility treatment possess an altered testicular metabolism profile that may hamper the nutritional support of spermatogenesis. Testicular biopsies from control (46, XY) (n = 6) and KS (47, XXY) (n = 6) individuals were collected and analyzed by proton high‐resolution magic‐angle spinning nuclear magnetic resonance spectroscopy. The mRNA and protein expression of crucial glycolysis‐associated enzymes and transporters were evaluated in parallel by quantitative PCR and Western blot, respectively. Our data revealed altered regulation of glucose transporters ( GLUT1 and GLUT3 ); phosphofructokinase 1, liver isoform ( PFKL ); and lactate dehydrogenase A ( LDHA ) expression in the testis of KS patients. Moreover, we detected a severe reduction in lactate and creatine accumulation within testicular tissue from KS men. The aberrant levels of the biomarkers detected in testicular biopsies of KS men may therefore be associated with the infertility phenotypes presented by these men. Mol. Reprod. Dev. 83: 208–216, 2016. © 2015 Wiley Periodicals, Inc .