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Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets
Author(s) -
Rao Shengbin,
Fujimura Tatsuya,
Matsunari Hitomi,
Sakuma Tetsushi,
Nakano Kazuaki,
Watanabe Masahito,
Asano Yoshinori,
Kitagawa Eri,
Yamamoto Takashi,
Nagashima Hiroshi
Publication year - 2016
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/mrd.22591
Subject(s) - myostatin , biology , transcription activator like effector nuclease , myogenesis , somatic cell nuclear transfer , somatic cell , microbiology and biotechnology , gene knockout , gene , nuclease , myocyte , skeletal muscle , genetics , genome , endocrinology , blastocyst , genome editing , embryogenesis
SUMMARY Myostatin ( MSTN ) is a negative regulator of myogenesis, and disruption of its function causes increased muscle mass in various species. Here, we report the generation of MSTN ‐knockout (KO) pigs using genome editing technology combined with somatic‐cell nuclear transfer (SCNT). Transcription activator‐like effector nuclease (TALEN) with non‐repeat‐variable di‐residue variations, called Platinum TALEN, was highly efficient in modifying genes in porcine somatic cells, which were then used for SCNT to create MSTN KO piglets. These piglets exhibited a double‐muscled phenotype, possessing a higher body weight and longissimus muscle mass measuring 170% that of wild‐type piglets, with double the number of muscle fibers. These results demonstrate that loss of MSTN increases muscle mass in pigs, which may help increase pork production for consumption in the future. Mol. Reprod. Dev. 83: 61–70, 2016. © 2015 Wiley Periodicals, Inc .